 The p185her2/neu-ECD DNA sequence is isolated from the CDNA of SKBR-3 tumor 
cell line by PCR. An EcoRI site is then removed by SOE PCR to make it assembly 
compatible. The 8 mutants are created by modifying the p185her2/neu-ECD DNA 
sequence using SOE PCR. Then the antigens will be fused with Mcherry.(also use 
SOE PCR)